Glut-1在良恶性骨肿瘤中的表达及临床意义

Glut-1在良恶性骨肿瘤中的表达及临床意义

樊健;俞光荣;袁锋;陈凯;梅炯;易祥华

【期刊名称】《同济大学学报（医学版）》

【年(卷),期】2013(034)003

【摘要】Objective To investigate the expression of glucose transporter protein-1 (Glut-1) in normal bone tissue,benign and malignant bone tumors and its clinical significance.Methods Immunohistochemical staining was applied to detect Glut-1 expression in 32 cases of bone osteosarcoma,10 cases of osteochondroma and 10 specimens of normal bone tissue.The expression of Glut-1 protein was also examined by Wester-blotting method in fresh specimens of osteosarcoma and normal bone tissue.Results Immunohistochemistry showed positive expression of Glut-1 in 31cases of osteosarcoma (96.9％),including strongly positive in 20 cases (62.5％) and weakly positive in 1 case.All osteochondroma specimens presented negative staining for Glut-1,except one case presenting weakly positive,while no positive expression of Glut-1 was detected in normal bone tissue.Westernblot showed the gray value increased significantly in 3 fresh specimens of osteosarcoma compared to normal bone tissue(P ＜0.05).Conclusion Glut-1 protein is positively expressed in osteosarcoma,which indicates that Glut-1 may be used to assess malignancy of bone tumors and as a potential target for treatment of bone tumors.%目的探讨葡萄糖转运蛋白