雅培RealTime HCV Genotype⒒(10)

Materials Required But Not Provided

Abbott RealTime HCV Genotype II Control Kit (List No. 08L21-80) Abbott m2000sp Instrument

Abbott mSample Preparation System (4 x 24 Preps) (List No. 04J70-24)

Abbott RealTime HCV Genotype II m2000 System Combined Application CD-ROM

Uracil-N-Glycosylase (UNG) (List No. 08L21-66) protocol

5 mL Reaction Vessels

200 mL Reagent Vessels

Transport Tubes (Master Mix Vials)

Abbott 96-Well Optical Reaction Plate

Abbott 96-Deep-Well Plate

Abbott Splash-Free Support Base

Abbott Optical Adhesive Cover

Abbott Adhesive Cover Applicator

Sample Racks

Round-bottom 11.6 to 16 mm Sample Tubes

Vortex Mixer

Centrifuge capable of 2000 g

Calibrated Precision Pipettes capable of delivering 20 μL-1000 μL

20 μL-1000 μL Aerosol Barrier Pipette Tips for precision pipettes

Molecular Biology Grade Water (RNase Free)

1.7 mL Molecular Biology Grade Microcentrifuge Tubes (Dot Scientific, Inc. or equivalent)

Cotton Tip Applicators (Puritan or Equivalent)

NOTE: If required per the Contamination From External dU-Containing Amplified Product section of this package insert.

NOTE: These items are used in the procedure for Monitoring the Laboratory for the Presence of Amplification Product. Refer to the QUALITY CONTROL PROCEDURES section of this package insert. Abbott m2000rt Instrument

Abbott RealTime HCV Genotype II m2000 System Combined Application CD-ROM

Abbott m2000rt Optical Calibration Kit (List No. 04J71-93)

Other Materials

Biological safety cabinet approved for working with infectious materials.

Sealable plastic bags

Procedural Precautions

Read the instructions in this package insert carefully before processing samples.

The Abbott RealTime HCV Genotype II Internal Control, Negative Control, and Positive Control vials are intended for single-use only and should be discarded after use.

Sample tubes should be inspected for air bubbles. If found, remove them with a sterile pipette tip. Reagent bubbles may interfere with proper detection of reagent levels in the reagent vessel, causing insufficient reagent aspiration, which could impact results. Caution should be taken to avoid cross-contamination between samples by using a new sterile pipette tip for each tube.

Use aerosol barrier pipette tips or disposable pipettes only one time when pipetting specimens or Internal

Control. To prevent contamination to the pipette barrel while pipetting, care should be taken to avoid touching