异丙酚对肝脏缺血再灌注损伤时的保护作用(4)

异丙酚对肝脏缺血再灌注损伤时的保护作用

hours-ischemia and reperfution .Campared with group B TNF-a decreased and IL-10increased significantly(P ＜0.05) in group C and group D.There is no significant difference between group C and group D(P＞0.05).

2. Hepatic NF-kB activation was induced in a time-dependent manner after

I/R.Propofol suppressed NF-kB activation.

3. Electron microscopic examination revealed that in group Bthere was a

significant increase in apoptotic hepatocytes and sinusoidal endothelial cell(SEC)after ischemia-reperfusion （P＜0.05）.Most of apoptotic cell were SEC and only a small number of hepatocytes. Mitochondria swelled obviously.Both SEC and hepatocytes apoptosis and mitochondria swell were much slighter in group C、D than in group B.

[Conclusions]In this study ,we can observed that cytokines and NF- kB activation increased,hepatocytes apoptosis and ultromicrostructure swelled during HIRI.These factors affect together.Propofol has some protective effect on HIRI in rats,such as 1. Propofol has protective effects on HIRI by inhibiting TNF-a and increasing IL-10 after hepatic ischemia reperfusion in rats.2. Hepatic is -chemia-reperfusion injury is related to the binding activation of NF- kB to special DNA sequence,propofol protects against hepatic ischemia-reperfusion by suppressing NF- kB activation.3.Propofol inhibits not only SEC and hepatocytes apoptosis but also mitochondria swell after ischemia-reperfusion through its antioxidant effect.

[Key words] Propofol ; ischemia-reperfusion ; liver ; cytokine ; ultromicrostructure ; NF- kB