胸腺肽β4在小鼠卯母细胞和早期胚胎中的表达与(6)

·1198·

生物化学与生物物理进展Prog.Biochem.Biophys.2009;36(9)

100滋m

Fig援5Tb4peptideexpressionindifferentstagesofembryonicdevelopmentandearlyimplantedblastula

Numbersnote:ThesameasFigure3.Lettersnote:A,2-cellembryos(early);B,2-cellembryos(latter);C,4-cellembryos;D,8-cellembryos(early);E,8-cellembryos(latter,densificated);F,Morular(early,approximate16cells);G,Morular(early,approximate32cells);H,Blastula(early);I,Blastula(latter);J,Earlyimplantedblastula.

Tb4蛋白在卵母细胞和早期胚胎的几个主要时期的相对表达强度如图6所示，在卵母细胞成熟过程中，各时期Tb4蛋白的荧光平均AIOD值无显著差异．而在胚胎发育过程中，从8细胞开始Tb4蛋白的表达量急剧增加．

2援3卵母细胞与胚胎发育过程中Tb4mRNA表达的RT鄄PCR检测结果

卵母细胞和胚胎发育过程中Tb4mRNA表达的RT-PCR检测结果见图7．

M1

Tb4220bpGAPDH307bp

2345

M1267891011-

+

Fig.7Tb4mRNAexpressioninmainstagesof

oocytesmaturationandembryonicdevelopment

1:G吁stage;2:Metaphase域ofoocytes(maturedinvivo);3:Metaphase域ofoocytes(maturedinvitro);4:GVBDblocked,IVC5h;5:Oocyteswithoutformingpolarbody,IVC12h;6:Zygotes;7:2-cellembryos;8:

4-cellembryos;9:8-cellembryos;10:Morular;11:Blastula;-:Negativecontrol;+:Positivecontrol(cDNAfrommouseliver).M:DL2000.

Fig.6RelativeexpressionofTb4peptide(2s)inmainstagesofoocytesmaturationandembryonicdevelopment

Numbersnote:1,GVstage;2,GVBDstage,IVC3h;3,GVBDblocked,IVC5h;4,Metaphase域ofoocytes(Maturedinvivo);5,Metaphase域ofoocytes(Maturedinvitro);6,Zygotes;7,2-cellembryos;8,4-cellembryos;9,8-cellembryos;10,Morular;11,Blastula.Thegroupswithsignificantdifference(P<0.05)werenotedwithdifferentlowercaseletters.

依据凝胶成像系统获得IOD数值进行统计分析，获得如下结果见图8：体内成熟卵母细胞中Tb4mRNA表达量均值略低于GV期，而体外成熟的表达量略高于GV期，但差异不显著．发